HPLC测定不同基原陈皮药材中橙皮苷含量

Content Determination of Hesperidin from Different Kinds of Pericarpium Citri Reticulatae by HPLC

目的:采用高效液相色谱法测定不同基原陈皮药材中橙皮苷含量,为科学评价及有效控制其质量提供可靠方法。方法:采用高效液相色谱法,Baseline C18色谱柱(4.60 mm×250 mm,5μm),流动相甲醇-水(1%乙酸溶液)(50∶50),检测波长283 nm,等度洗脱,流速1.0 mL·min-1,柱温室温,进样量20μL。结果:橙皮苷质量浓度在0.002～0.040 g·L-1呈良好的线性关系(r=0.999 9),平均回收率102.3%(n=9),RSD 1.6%。含量测定结果表明,同一产地不同基原陈皮药材中橙皮苷含量存在显著差异。结论:该方法简单、可行,适用于不同基原陈皮药材中橙皮苷的含量测定以及质量评价。

Objective：To determine the content of the hesperidin in six different kinds of pericarpium citri reticulatae and gained a method to estimate and control the quality of medical material and effectively.Method： The HPLC was adopted to do this examination,the separat on was performed on a Baseline C18 column（4.60 mm×250 mm,5 μm）,methanol-water（1% acetic acid solution）（50∶50） was used as the mobile phase with isocratic elution,the detective wavelength was at 283 nm,the flow rate was 1.0 mL· min-1,the column temperature was room temperature and the injecti on volume was 20 μL.Result： The hesperidin showed a good linearity in the range of 0.002-0.040 g·L-1（r=0.999 9）,the average recovery was 102.3%（n=9）,RSD 1.6%.The contents of hesperidin in peels of citrus was varied from different species.Conclusion： The method is simple and available,which can be used to determine and estimate hesperidin in different kinds of pericarpium citri reticulatae.