高效毛细管电泳法测定加味逍遥丸中芍药苷、栀子苷的含量

Determination of Paeoniflorin and Geniposide in Jiawei Xiaoyao Pills by High Performance Capillary Electrophoresis

目的:建立加味逍遥丸中芍药苷、栀子苷同时测定的高效毛细管电泳分析方法。方法:采用高效毛细管电泳法,缓冲体系为25 mmol·L-1硼砂缓冲液(NaOH调pH 10.5),熔融石英毛细管(60 cm×50μm),运行电压25 kV,柱温25℃,检测波长230 nm。结果:芍药苷、栀子苷分别在0.008 1～0.162 6 g·L-1和0.023 8～0.475 6 g·L-1线性关系良好(r=0.999 8和0.999 7),样品平均加样回收率为99.67%(RSD 1.27%)和99.36%(RSD 1.53%)结论:该方法简单、快速准确、重复性好,适用于加味逍遥丸的质量控制。

Objective： To establish a new high performance capillary electrophoresis （HPCE） method for determination of paeoniflorin and geniposide in Jiawei Xiaoyao pills. Method： Paeoniflorin and geniposide in Jiawei Xiaoyao pill were determined by using HPCE. The background electrolyte system selected was 25 mmol. L- sodium borate buffer （pH was adjusted to 10.5 by caustic soda）. Capillary electrophoresis was performed using 60 cm ×50 μm fused silica capillary column, applied voltage was 25 kV; temperature was 25 ℃. The detection wavelength was set at 230 nm. Result： The linear range of paeoniflorin and geniposide was 0. 008 1-0. 162 6 g · L^-1 and 0.023 8-0.475 6 g · L^-1 （r =0.999 8 and 0.999 7）, and the average recovery was 99.67% （RSD 1.27% ） and 99.36% （RSD 1.53% ）. Conclusion： The method is simple, rapid and reproducible, and could be used for the quality control of Jiawei Xiaoyao pills.