比色法测定大蒜多糖提取物中总多糖含量

Content Determination of Total Polysaccharide in the Garlic Polysaccharide by Colorimetry

目的:建立大蒜多糖提取物中总多糖的含量测定方法。方法:以葡萄糖为对照品,硫酸-苯酚比色法测定大蒜多糖提取物中总多糖含量。结果:样品经苯酚-硫酸显色后于485 nm处测定吸收度,在此波长处溶液的吸光度与葡萄糖含量呈良好的线性关系;线性范围0.0116～0.0578 g·L-1(r=0.999 5),加样回收率101.4%,RSD 2.1%。结论:该方法简单,准确性、重复性良好,可作为大蒜多糖提取物中总多糖质量的控制方法。

Objective： To develop a method for content determination of total polysaccharide in garlic polysaceharide. Method： Glucose was used as a reference substance, and phenol-sulfuric acid was applied as coloring reagent to determine the content of total polysaccharide. Result： The calibration curve of glucose showed good linearity in the range of 0. 011 6-0. 057 8 g · L-1 （r =0. 999 5）. The average recovery rate was 101.4% , and RSD was 2. 1%. Conclusion： The determination method is simple, sensitive and accurate, which can be used for evaluating the quality of total polysaccharide in the garlic polysaccharide.