细柱五加果实提取物对免疫抑制小鼠的免疫调节作用

The Immunoregulation of Extracts from Fruits of Acanthopanax gracilistylus in Immunosuppressive Mice

目的:研究细柱五加果实乙醇提取物对免疫抑制小鼠的免疫调节作用。方法:将小鼠随机平均分为细柱五加果实的高、中、低3个剂量组(2.8,1.4,0.7 g·kg-1)、阳性对照组(20 mg·kg-1左旋咪唑)、空白对照组和模型对照组(各组n=10)。各组小鼠均ig给药(20 mL·kg-1)。连续10 d。空白对照组和模型对照组均ig同等剂量的蒸馏水。从第7天开始,除空白对照组外,其余各组小鼠均ip环磷酰胺(80 mg·kg-1)以制作免疫抑制小鼠模型。连续4 d。采用免疫器官质量法和碳粒廓清法,以脾脏指数、胸腺指数、碳粒廓清指数及校正吞噬指数为指标来评价细柱五加果实乙醇提取物对免疫抑制小鼠非特异性免疫功能的影响;采用血清溶血素测定法,以血清溶血素含量为指标来评价细柱五加果实乙醇提取物对免疫抑制小鼠特异性免疫功能的影响。结果:细柱五加果实乙醇提取物对免疫抑制小鼠的脾脏指数和胸腺指数无显著性影响,但可以提高其碳粒廓清指数、校正吞噬指数和血清溶血素含量(P<0.05或P<0.01)。结论:细柱五加果实乙醇提取物对免疫抑制小鼠的非特异性免疫功能和特异性体液免疫功能均有提高作用。

Objective： To study the immunoregulation of the ethanol extracts from fruits of Acanthopanax gracilistylus in immunosuppressive mice. Method： The mice were randomly divided into the fruits of A. gracilistylus high dose group, middle dose group, low dose group （2.8, 1.4, 0.7 g· kg^-1 ） , positive control group, control group and model control group. Each group was administrated intragastrically at 20 mL-kg-1 for 10 days （n = 10）. The control group and model control group were administrated intragastrically with the same dose of distilled water. From the 7ta day, except the control group, other groups were injected cyclophosphamide （80 mg. kg-1） intraperitoneally to produce the immunosuppressive mice model for 4 days. The weight of immune organs and the carbonic particle clearance methods were used to evaluate the effects of the ethanol extracts on non-specific immune function in immunosuppressive mice by spleen index, thymus index, carbonic particle clearance index and corrected phagocytic index; the serum hemolysin examination was used to evaluate the effects of ethanol extracts by serum hemolysin index. Result： The ethanol extracts had no significant impacts on spleen index and thymus in immunosuppressive mice, but could improve carbonic particle clearance index, corrected phagocytic index and serum hemolysin index of immunosuppressive mice （P 〈 0.05 or P 〈 0.01 ）. Conclusion ： The ethanol extracts of the fruits of A. gracilistylus can enhance non-specific immune function and specific humoral immune function in immunosuppressive mice.