摘要
目的:检测PTEN、STAT3和CyclinD1蛋白在宫颈癌组织中的表达。方法:收集2009年1月～2011年1月经手术治疗的宫颈癌手术切除标本85例,所选病例按照TNM分期分为Ⅰ、Ⅱ、Ⅲ期,另取因子宫肌瘤而行子宫切除的正常宫颈上皮组织作为对照组;免疫组化法检测宫颈组织中PTEN、STAT3和CyclinD1蛋白的表达。结果:PTEN蛋白在宫颈癌组织中的阳性率为42.4%,明显低于正常对照组的95.0%(P<0.01);而STAT3和CyclinD1蛋白在癌组织中的阳性率分别为71.8%和83.5%,明显高于正常宫颈组织的15.0%和25.0%(P<0.01)。在3个临床分期中,PTEN、STAT3和CyclinD1蛋白的表达率均存在统计学差异(P<0.01),PTEN蛋白的表达量会随着临床分期的增加而减少,但STAT3和CyclinD1蛋白随着临床分期的增加其表达率也相应升高。结论:PTEN蛋白的表达降低和STAT3、CyclinD1蛋白的表达增加在宫颈癌的发生发展中起到一定的作用,可以用于肿瘤良、恶性的鉴别诊断、病理分级以及预后判断等方面。
Objective：To detect the expressions of PTEN,STAT3,and CyclinD1 in cervical cancer tissue. Methods：Eighty-five cervical cancer samples after surgical therapy from January 2009 to January 2011 were collected,then they were divided into stage Ⅰ,stage Ⅱ,and stage Ⅲ according to TNM staging;normal cervical epithelial tissue samples after uterine resection because of hysteromyoma were selected as control group.Immunohistochemical method was used to detect the expressions of PTEN,STAT3,and CyclinD1. Results：The positive expression rate of PTEN in cervical cancer tissue was 42.4%,which was significantly lower than that in normal control group（95.0%）（P0.01）;the positive expression rates of STAT3 and CyclinD1 in cervical cancer tissue were 71.8% and 83.5%,respectively,which were significantly higher than those in normal cervical tissue（15.0% and 25.0%,respectively）（P0.01）.There was significant difference in the expression rates of PTEN,STAT3,and CyclinD1 among the cervical cancer samples of stage Ⅰ,stage Ⅱ,and stage Ⅲ（P0.01）,the expression level of PTEN decreased with the increase of clinical stage,but the expression rates of STAT3 and CyclinD1 increased with the increase of clinical stage. Conclusion：The down-regulation of PTEN and up-regulation of STAT3 and CyclinD1 play important roles in the occurrence and development of cervical cancer,which can be used for differential diagnosis,pathological grades,and predicting prognosis of cervical tumors.
出处
《中国妇幼保健》
CAS
北大核心
2012年第15期2344-2346,共3页
Maternal and Child Health Care of China
