摘要
目的:建立中药挥发油中桉油精含量的HPLC检测方法。方法:采用Dikma Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水(64∶36),流速1.0 mL·min-1,检测波长203 nm,柱温30℃。结果:桉油精进样量在4.72~47.2μg范围内具有良好的线性关系(r=0.9996);平均回收率为99.3%,RSD为0.86%。结论:该方法简便快速、准确,具有良好的重复性和回收率,可作为中药挥发油中桉油精的定量分析方法。
Objective:To establish an HPLC method for the content determination of eucalyptol.Methods:The separation was performed on a Dikma Kromasil C18(250 mm×4.6 mm,5 μm)column.The mobile phase was acetonitrile-water(64∶36)at the flow rate of 1.0 mL·min-1.The detective wavelength was set at 203 nm,and the column temperature was 30 ℃.Results:The calibration curve was linear in the range of 4.72-47.2 μg(r=0.9996).The average recovery was 99.3% with RSD of 0.86%.Conclusion:This method is simple,rapid,accurate and with good repeatability and recovery,and can be used as a quantitative analysis method for eucalyptol in herbal volatile oil.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第5期769-771,共3页
Chinese Journal of Pharmaceutical Analysis
基金
四川省教育厅重点项目(08ZA114)
四川省卫生厅科研课题(090190)
泸州市指导性项目(泸市科[2007]64号)
关键词
高效液相色谱
中药提取物
挥发油
桉油精
香料
防腐剂
含量测定
HPLC
extract of traditional Chinese medicine
volatile oil
eucalyptol
perfume
preservative
assay