HPLC测定11种中药挥发油中桉油精的含量

Determination of eucalyptol in 11 kinds of herbal volatile oil by HPLC

目的:建立中药挥发油中桉油精含量的HPLC检测方法。方法:采用Dikma Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水(64∶36),流速1.0 mL·min-1,检测波长203 nm,柱温30℃。结果:桉油精进样量在4.72～47.2μg范围内具有良好的线性关系(r=0.9996);平均回收率为99.3%,RSD为0.86%。结论:该方法简便快速、准确,具有良好的重复性和回收率,可作为中药挥发油中桉油精的定量分析方法。

Objective：To establish an HPLC method for the content determination of eucalyptol.Methods：The separation was performed on a Dikma Kromasil C18（250 mm×4.6 mm,5 μm）column.The mobile phase was acetonitrile-water（64∶36）at the flow rate of 1.0 mL·min-1.The detective wavelength was set at 203 nm,and the column temperature was 30 ℃.Results：The calibration curve was linear in the range of 4.72-47.2 μg（r=0.9996）.The average recovery was 99.3% with RSD of 0.86%.Conclusion：This method is simple,rapid,accurate and with good repeatability and recovery,and can be used as a quantitative analysis method for eucalyptol in herbal volatile oil.