响应面法优化莲子总蛋白提取工艺的研究

Optimization of Extraction Technology of Lotus Seed Total Protein by Response Surface Methodology

为优化莲子总蛋白的提取工艺,首先确定了最佳提取液为Tris-HCl缓冲液;在单因素试验的基础上,采用Box-Behnken试验设计方法,研究液料比、提取时间和提取温度对莲子总蛋白提取率的影响,建立总蛋白提取率的二次回归方程,并对其工艺参数进行优化。结合实际操作的研究结果表明:提取莲子总蛋白的最佳工艺参数为液料比7.2∶1,提取时间2.4 h,提取温度11.1℃,总蛋白的提取率达到81.35%,与回归模型的相对误差小于0.5%。

The extraction of total protein from lotus seed was studied. Tris-HC1 buffer was selected as the best extract. According to the single-factor experiments, three levels of the factors （the ratio of buffer to solid, extraction time and temperature） were selected for the Box-Behnken factorial design to establish a quadric regression equation for describing the yield of total protein. By response surface analysis and the actual operation, the optimum extraction conditions were obtained as follows： the ratio of buffer to solid of 7.2：1, extraction time of 2.4 h and extraction temperature of 11.1℃, the extraction yield of total protein was 81.35%, resenting a relative error of less than 0.5 %.