百合总皂苷提取工艺及抗抑郁活性研究

Extraction and Purification Techniques and Antidepression Activity of Total Saponins from Bulbs of Lilium brownii

本文研究百合总皂苷最佳提取工艺,继而采用AB-8大孔吸附树脂富集纯化百合总皂苷,并测试其抗抑郁活性。采用正交实验,考察乙醇浓度、提取次数、固液比及提取时间四个因素对该工艺的影响;采用小鼠悬尾实验和小鼠强迫游泳实验评价百合总皂苷的抗抑郁活性。确立了百合总皂苷的最佳提取工艺为:提取时间3h,乙醇浓度80%,固液比1∶10,提取次数为3次。在最佳提取工艺条件下,总皂苷提取率为1.24%。经过AB-8大孔树脂富集纯化总皂苷的含量达到62%以上。药理实验表明,百合总皂苷中剂量、小剂量能明显缩短小鼠悬尾的不动时间和游泳时间(P<0.05或者P<0.01),百合富集纯化的总皂苷部位具有较好的抗抑郁活性。

Extraction and purification techniques of total saponins from bulbs of Lilium brownii F. E. Brown var. viridu- lum Baker. and the antidepressant activity of the total saponins were studied in this paper. Based on orthogonal design, four factors in extraction of saponins from bulbs of Lilium brownie were optimized： concentration of ethanol, reflux time, solid/liquid ratio and extraction times. The optimized process for extracting total saponins from bulbs of Lilium brownii was confirmed as follows：reflux time,3 h; alcohol,80% ;solid/liquid, 1 ： 10;extraction 3 times. The highest extraction rate reaches 1.24%. Then,the extract of total saponins was separated and purified by AB-8 porous resin absorption and the total saponins content reaching 62%. In antidpression effect filtration study, the mouse tail suspended experiment, mouse forced swimming experiment were performed, the result showed that the total saponins was the main antidepression component. The above results showed that the total saponins had certain antidepressant activity.