纤维素酶提高红三叶草醇提物体外抗氧化活性的研究

Enhanced in vitro antioxidant activity of red clover extracts using cellulase

红三叶草粉末经70%乙醇室温提取、43℃减压浓缩,过大孔吸附树脂柱,再先后用蒸馏水和70%乙醇洗脱,收集70%乙醇洗脱液,减压浓缩得红三叶草醇提物。为了提高红三叶草醇提物体外抗氧化活性,将红三叶草醇提物与纤维素酶在50℃恒温条件下反应30min,比较酶解前后红三叶草醇提物的清除DPPH自由基能力和还原力。结果表明:在相同的浓度下,经纤维素酶处理后的红三叶草醇提物抗氧化能力要高于未被处理的红三叶草醇提物。

Red clover powder was extracted with 70% ethanol at room temperature.The extracted solution was concentrated by evaporation under reduced pressure at 43 ℃,and chromatographed by a macro reticular absorption resin column.Red clover extracts was obtained by concentrating the fraction eluted by 70% ethanol at the same condition.To increase the antioxidant activity,red clover extracts was incubated with cellulase at 50 ℃ for 30 min.The DPPH free radical scavenging capacity and reducing power of red clover extracts before hydrolysis by cellulase are compared with those after hydrolysis by cellulase.The results showed that the antioxidant activity of cellulase-treated red clover extracts was higher than those of untreated red clover extracts at the same concentration.