发酵乳中双岐杆菌种类快速识别

Rapid identification bifidobacterium in fermented milk products by PCR-DGGE analysis

建立发酵乳制品中双歧杆菌快速识别方法。采用酶解前处理法获取样品中菌体细胞,基于PCR-DGGE技术确定发酵乳中双歧杆菌种属。该方法能准确、快速鉴别双歧杆菌,检出限为105cfu/mL。该方法可用于发酵乳中双歧杆菌的准确识别。

To establish a rapid identification method for bifidobacterium in fermented milk products.The use of Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis（PCR-DGGE） technique in identifying bifidobacterium present in commercial probiotic yoghurts dealed with pronase E was evaluated.Identification was achieved comparing the PCR-DGGE patterns obtained from the analysed products with the ladder bands.PCR-DGGE assays enabled identification of the species of bifidobacterium initially present in commercial fermented milk products and their accurate quantification with a detection threshold of 105 cells per mL of product.Established PCR-DGGE analysis method is situble for detection of bifidobacterium in commercial probiotic yoghurts.