青钱柳愈伤组织增殖

Proliferation of Cyclocarya paliurus Callus

为获得大量优质愈伤组织,以青钱柳(Cyclocarya paliurus)叶片为外植体诱导出的愈伤组织为材料,研究了基本培养基、蔗糖质量浓度、植物生长调节剂和镧稀土对愈伤组织增殖的影响。结果表明,综合愈伤组织增长率以及愈伤组织的颜色和状态因素,以MS基本培养基的增殖效果最佳;蔗糖质量浓度为20.00 g/L时愈伤组织增长率最高;植物生长调节剂对愈伤组织增殖的最佳组合为KT0.20 mg/L+2,4-D0.20 mg/L,平均增长量、增长率分别为4.38 g/瓶和1 024.51%。稀土对愈伤组织增殖的最佳质量浓度为0.50 mg/L。研究结果为进一步建立青钱柳悬浮培养体系和次生代谢调控的研究提供基础,同时也为青钱柳组培快繁技术的研究提供依据。

In order to obtain a large amount of high quality callus,leaves of Cyclocarya paliuru were used as explants and induced calluses were used as research materials.Effects of basic medium,sucrose content,plant growth regulator and rare earth on proliferation of callus were studied.Results showed that the optimal basic medium for proliferation was MS judged from growth rate,color and status of callus.The growth rate of callus was the highest by adding 20g/L sugar to the medium.KT 0.2mg/L＋2,4-D 0.2mg/L was the best combination of plant growth regulators,whose average increment and growth rate were 4.38g per bottle and 1024.51%,respectively.Rare earth La could promote callus proliferation,and the optimal concentration of La（NO3）3 was 0.5mg/L.