RP-HPLC法测定人精液中维生素E的含量

Determination of Vitamin E in Human Semen by RP HPLC

目的 :建立测定人精液维生素E含量的反相高效液相色谱法。 方法 :用正己烷萃取人精液维生素E ,氮气吹干 ,以甲醇定容 ;以维生素E作外标 ,用紫外检测仪检测 ,波长为 2 0 5nm。 结果 :线性关系为y =0 .30 2 9+0 .0 0 730x,r=0 .9994,回收率在 85 %～ 115 %之间 ,检测限为 4.5 35× 10 -2 μmol/L ,测定 1份样品约需 12min。 结论 :本法与比色法或荧光法比较 ,测定人精液中维生素E含量准确 ,特异性强 ,灵敏度高 ;结果提示不育病人精子密度越高精液维生素E含量越高。

Objectives:A RP HPLC method to determine vitamin E extracted directly from human semen with n hexane was developed. Methods:The dry residue was dissolved in methanol.Vitamin E used as external standard and samples were detected by UV Vis detector. Results:A standard curve for linearity was y=0.3029+0.00730x and the correlation coefficient was higher than 0.999 in the concentration range of (2.375～38.820) μmol/L.The recovery was 85% to 115% and the lowest detection limit of vitamin E was 0.04535 μmol/L.12 minutes were required for each analysis. Conclusions:Compared with the colorimetric method and fluorometric method,this method permits more sensitive,selective and precise determination of vitamin E in human semen.