NDRG4基因甲基化检测在结直肠癌早期诊断中的应用研究

Study of early diagnosis of colorectal cancer by analysis of NDRG4 gene methylation

目的探讨NDRG4甲基化检测在结直肠癌诊断中的价值。方法从84例结直肠癌手术切除癌组织、癌旁组织及其术前粪便、尿、血中提取DNA,应用巢式甲基化特异性PCR(nested methylation specific PCR,nMSP)检测结直肠癌粪便、尿、血中NDRG4基因甲基化水平。比较结直肠癌组织和粪便、尿、血中NDRG4甲基化检测在结直肠癌诊断中的敏感性和特异性。结果在84例结直肠癌中,癌组织NDRG4检出率为81.0%,癌旁组织检出率为8.3%,敏感性为81.0%,特异性为91.7%;结直肠癌中粪便NDRG4基因甲基化的敏感性和特异性在3个微量DNA中最高,尿次之。结论结直肠癌组织NDRG4基因甲基化异常发生率高;粪便和尿中NDRG4甲基化异常可作为结直肠癌早期诊断的肿瘤标志物。

Objective To investigate the values of diagnosis of colorectal cancer by analysis of NDRG4 methylation.Methods The NDRG4 gene methylation were detected by nested methylation specific PCR（nMSP） in 84 colorectal cancer tissues,para-cancer tissues,stool DNA,urine DNA and blood DNA.The sensitivity and specificity of NDRG4 methylation marker in colorectal cancer diagnosis were analyzed.Results Among 84 colorectal cancers,NDRG4 gene methylation was found in 81.0% of cancer tissues and 8.3% of para-cancer tissues respectively.The sensitivity and specify of NDRG4 for diagnosis of colorectal cancer were 81.0% and 91.7% respectively,and the stool and urine NDRG4 better sensitivitily and specificitily in diagnosis of colorectal cancer.Conclusion The abnormal methylation of NDRG4 is happened frequently in colorectal cancer;hypermethylaion of NDRG4 gene mehylation in stool and urine DNA can be used as candidates of tumor marker for early diagnosis of colorectal cancer.