烟酸抑制血管紧张素Ⅱ诱导的内皮细胞C反应蛋白的表达

Niacin inhibits C-reactive protein expression induced by angiotensin Ⅱ in human endothelial cells

目的探讨烟酸对血管紧张素Ⅱ诱导的人脐静脉内皮细胞(HUVECs)C反应蛋白表达的影响。方法分别使用不同浓度烟酸(0.25、0.5或1.0 mM)预处理HUVECs不同时间(1、2、6、12及24 h)后,使用血管紧张素Ⅱ(1μmol/L)诱导HUVECs表达C反应蛋白。荧光实时定量PCR(RT-PCR)检测CRP mRNA的变化。Western Blot检测CRP蛋白和NF-κB p65蛋白的变化。结果烟酸能抑制血管紧张素Ⅱ诱导的HUVECs中CRP mRNA和蛋白的表达,而且这种抑制呈时间和浓度依赖性。HUVECs在使用1 mmol/L烟酸预处理24 h后,AngⅡ+Niacin组与AngⅡ组相比,NF-κB蛋白表达有明显下降,同时CRP蛋白表达也同步下降。结论烟酸能抑制血管紧张素诱导的HUVECs中CRP mRNA及蛋白的表达,抑制NF-κB是可能的机制之一。

Objective To investigate the effect of niacin onC-reactive protein expression induced by angiotensinIIin human umbilical vein endothelial cells（HUVECs）.Methods After HUVECswere pretreated with different concentrations of niacin（0.25,0.5,1.0 mM） for differenttime（1,2,6,12,24 h）,HUVECs were treated with angiotensionⅡ（1 μmol/L） for 12 h.Realtime RT-PCR was used to detect changes of CRP mRNA level.Western blot analysis was used to detect changes of CRP and NF-κB p65 protein level.Results In HUVECs,Niacin inhibited C-reactive protein expression induced by angiotensinIIin time-and concentration-dependent way.The further study indicated that niacin has the ability to inhibited NF-κB p65 protein expression in HUVECs.Conclusion Niacin can decreaceC-reactive protein expression induced by angiotensinIIin HUVECs,and the possible mechanismis the inhabitation of NF-κB.