摘要
建立了固相萃取-高效液相色谱-串联质谱法测定鸡肉中泰妙菌素残留的分析方法。试样经磷酸盐缓冲液（pH8.0）-乙酸乙酯混合提取,经水饱和正己烷除脂后,过MCX固相萃取小柱净化,采用HPLC-MS/MS电喷雾电离正离子扫描,多反应监测模式（MRM）检测,内标法定量。泰妙菌素在0.5~50μg/L的浓度范围内呈现良好的线性关系,相关系数R2大于0.999。其检测限为0.25μg/kg,定量限为0.5μg/kg。
A high performance liquid chromatography-tandem mass spectrometry （ HPLC-MS/MS ） method was developed to determine tiamulin residues in chicken meat by solid phase extraction （ SPE ） . The sample was extracted by phosphate buffer （ pH 8.0 ） -ethyl acetate mixture solvent. After removing fat by water-saturated n-hexane , the sample was cleaned up by SPE using an oasis MCX extraction catridge. Tiamulin was detected by HPLC-MS/MS with MRM mode. It showed a good linearty in the range of 0.5 to 50 μg/L. The limit of detection was 0.25 μg/kg and the limit of quantification was 0.5 μg/kg.
出处
《中国家禽》
北大核心
2012年第11期28-31,共4页
China Poultry
