乌司他丁对急性创伤患者血清肿瘤坏死因子、白细胞介素-8、白细胞介素-10浓度的影响

Effects of Ulinastatin on serum TNF,IL-8,IL-10 concentration of acute wounds patients

目的观察乌司他丁对急性创伤患者血清肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)、白细胞介素-10(IL-10)浓度的影响。方法 46例急性创伤患者随机分成A、B两组,A组给予常规清创、抗感染等处理,B组在常规清创、抗感染等处理的基础上,加用乌司他丁治疗。分别在治疗前及治疗后第1、3、5天取外周静脉血,采用ELISA法检测血清中的TNF-α、IL-8、IL-10水平。同时将20例健康体检者作为对照C组。结果治疗前A、B两组血清TNF-α、IL-8浓度比较差异无统计学意义(P>0.05),治疗后第3、5天B组血清中TNF-α、IL-8浓度明显低于A组(均P<0.05),且B组血清中IL-10浓度第3、5天明显高于A组(均P<0.05)。结论乌司他丁能抑制创伤患者血清中TNF-α、IL-8的释放,促进抗炎因子IL-10的释放,从而有效降低创伤患者的炎症反应。

Objective To study the correlation between Ulinastatin and serum levels of TNF-α,IL-8 and IL-10 in patients with acute wounds.Methods 46 patients with acute wounds were randomly divided into two groups.Patients in A group was treated with conventional anti-inflammatory treatment,while patients in group B was treated with Ulinastatin on the basis of the conventional treatment.Peripheral blood were collected before treatment and after treatment on the one,three and five day.Serum levels of TNF-α,IL-8 and IL-10 were determined by ELISA.20 healthy persons served as control.Results Serum level TNF-α and IL-8 in A and B two groups of patients were significantly higher than group C（P 0.05）.Serum level of TNF-α and IL-8 level before treatment in A and B groups were not significantly（P 0.05）.Serum level of TNF-α and IL-8 in group B on day three and five were lower than in group A（P 0.05）,and serum level of IL-10 in the group B were higher than group A（P 0.05）.Conclusion Ulinastatin can inhibit release of TNF-α、IL-8 on serum in trauma patients,and promote release of anti-inflammatory cytokine IL-10,can effectively reduce inflammatory response of the trauma patients.