新疆棕色棉纤维中原花青素的提取及鉴定

Extraction and Identification of Proanthocyanidins from Brown Cotton Fibers

选取新疆5个棕色棉品种(系),分别为棕86、新彩棉1号、新彩棉5号、棕196和棕470,它们的纤维色泽由深到浅依次为棕86>新彩棉5号>棕196>新彩棉1号>棕470。以普通白色棉品种为对照,用含体积分数为0.1%三氟乙酸的乙腈水溶液(乙腈水溶液的体积分数为50%)分别冷浸提取各品种(系)的棉纤维色素,通过紫外光谱吸收和铁盐催化法鉴定出棕色棉和白色棉纤维提取液中均含有原花青素;利用铁盐催化反应比色法定量测定棕色棉和白色棉纤维中原花青素含量。结果显示,棕色棉纤维中原花青素含量明显高于白色棉;除棕470外,棕色棉纤维的色泽同原花青素的含量明显相关,棕色棉纤维的色泽越深,其原花青素的含量越高。可见,原花青素是棕色棉纤维色素的一种重要组分。

In this research five brown-fiber cotton cultivars（lines）（Gossypium hirsutum L.）,Zong 86,Xincaimian 5,Zong 196,Xincaimian 1,Zong 470 and one white-fiber cultivar as control were analyzed for the components of pigments in Xinjiang.The order of brown-fiber color strength was as follow： Zong 86〉 Xincaimian 5 〉Zong 196〉 Xincaimian 1 〉Zong 470.The pigments in both brown and white fibers were extracted by φ=50% Methyl cyanide（containing φ=0.1% Trifluoroacetic acid） at room temperature.Proanthocyanidins content in the φ=50% Methyl cyanide（containing φ=0.1% Trifluoroacetic acid） extractive solution was analysed by UV spectrum and ferric salts catalyst method.Furthermore,the proanthocyanidins concentration was determined using ferric ions colorimetry.The results showed that the proanthocyanidins concentration in the fibers of all the five brown-colored cultivars was much higher than that in the white fiber cultivar;except for Zong 470,the color strength of colored cotton fibers is significantly correlated with the proanthocyanidins concentration.The deeper the color of brown fibers is,the more proanthocyanidins they contain.This study indicates that the procyanidins is one important component of the pigments in brown-colored cotton fibers.