脉冲电场和pH值协同作用对卵白蛋白微观结构的影响

Effect of PEF Treatment on Secondary Structure of Ovalbumin under Various pH Conditions

采用自行研制的高强脉冲电场设备,利用远紫外圆二色光谱研究脉冲电场对卵白蛋白二级结构的影响。结果表明:多循环脉冲电场处理(26.7kV/cm)可导致卵白蛋白二级结构展开,当实验体系pH值分别为3、7和12时,4次循环(脉冲时间192μs)脉冲电场作用诱使卵白蛋白α-螺旋结构展开度分别为25.43%、23.99%和42.20%,β-折叠结构展开度分别为26.20%、15.99%和38.53%。

The effect of high intensity pulsed electric field（HIPEF） treatment on the secondary structure of ovalbumin under various pH conditions was explored using circular dichroism（CD） spectroscopy.The results indicated that 4 circles of PEF treatment（192μs） at the field intensity of 26.7 kV/cm could induced the unfolding of α-helix structure by 25.43%,23.99% and 42.20%,as well as unfolding of β-sheet structure by 26.20%,15.99% and 38.53% under the conditions of pH 3,7 and 12,respectively.