AuRu纳米粒子修饰的辣根过氧化物酶传感器研究

Modification of Horseradish Peroxidase Sensor by AuRu Nanoparticles

采用反向胶束法在室温下合成出AuRu纳米粒子,使用X射线衍射(XRD)、能量色散X射线光谱(EDS)、高分辨透射电镜(HRTEM)表征其结构、组成和形貌,制备其修饰辣根过氧化物酶传感器(HRP/AuRu/GC)。使用循环伏安法(CV)和计时电流法(i-t)研究该传感器对H2O2的检测性能。结果表明:HRP/AuRu/GC对H2O2检测具有高的灵敏度和稳定性,在H2O2浓度为1×10-7～1×10-3mol/L范围内检测电流与浓度的负对数成线性关系,线性相关系数R2=0.9959,检出限为5.02×10-8mol/L。电极使用1个月后其响应电流为原来的91.54%。

AuRu nanoparticles were synthesized by a reverse microemulsion method.The structure,composition,and morphology of AuRu were characterized by X-ray diffraction（XRD）,energy dispersive X-ray spectroscopy（EDS） and high-resolution transmission electron microscopy（HRTEM）.A novel biosensor was prepared by using AuRu nanoparticles to modify horseradish peroxidase（HRP）.The performance of the developed sensor for determining hydrogen peroxide（H2O2） were evaluated by cyclic voltammetry（CV） and chronoamperometry.The results showed that HRP/AuRu/GC had high sensitivity and stability of determination for H2O2.The ccurrent revealed an excellent linear relationship with the negative logarithm of concentration in the range of 1 × 10-7-1× 10-3 mol/L with a linear coefficient of 0.9959.The detection limit for H2O2 was 5.02 × 10-8 mol/L.The current of electrode response still retained 91.54% of the original level after 30 days of storage.