东北林蛙卵蛋白酶解工艺及其酶解物的抗疲劳活性

Enzymatic Hydrolysis and Anti-Fatigue Activity of Egg Protein of Rana dybowskii

研究复合蛋白酶对东北林蛙卵蛋白的酶解工艺及其酶解物的抗疲劳活性。分别考察pH值、加酶量、固液比、反应时间、反应温度对林蛙卵蛋白水解度的影响,并通过正交试验得到最佳酶解工艺条件:加酶量3000U/g pro、温度55℃、pH8.0、水解时间5h、固液比1:5.5(m/V)。以昆明小鼠为实验对象,通过测定小鼠的负重游泳时间、肝糖原及血乳酸含量,研究东北林蛙卵蛋白酶解物的抗疲劳活性,结果显示最佳优化条件下得到的林蛙卵蛋白酶解物具有较好的抗疲劳作用。

In order to exploit the resource of Rana dybowskii eggs, protamex was used to hydrolyze egg protein into small peptides in this study. The degree of hydrolysis of Rana dybowskii eggs was investigated with respect to hydrolysis parameters such as pH, enzyme dose, solid-to-liquid ratio, hydrolysis time and temperature. The optimal hydrolysis parameters were determined as follows： pH 8.0, 3000 U/g pro of enzyme dose, 55 ℃, 1：5.5 （m/V） of solid-to-liquid ratio and 5 h of hydrolysis time. The hydrolysate obtained under these conditions was determined to have obvious anti-fatigue function in mice in terms of weight-loaded swimming time and the contents of blood lactic acid and liver glycogen.