纳豆菌特性的研究

Research on biological characteristics of natto Strain

筛选具有强溶栓活性等多种生物学功能的纳豆菌,并对其形态、生长特性等进行研究。分离出单菌落,进行菌种鉴定,并在不同条件下用测菌悬液吸光度的方法研究其生长特性。经菌落形态、菌体形态以及生理生化特性鉴定确定为枯草杆菌。该菌种在种子培养基中生长的最适pH值为6.0～8.0,生长温度为30～45℃,耐盐性较高,在含7%以下NaCl的盐溶液中均能生长。纳豆菌的生长条件适宜于下一步产纳豆激酶的研究。

Natto strains with multiple biological functions such as strong thrombolytic activity were screened out for research on the forms and growth features. Single colonies were separated for identification of the strains and then the growth features were studied by methods for measuring suspension absorption in different conditions. Identifica- tion of the forms of colonies and thallis and the physiological-biochemical characteristics determined it as hay bacil- lus. The best growth environments for the hay bacillus in culture medium are pH 6.0 - 8.0 and 30 - 45 ℃ with the characteristics of high Salt-tolerance. The hay bacillus can grow in 7% NaCL The growth environments for Natto strains are applicable to the study of the producing of Bacillus subtilis.