自发性高血压大鼠恐惧情绪与内皮舒张功能障碍的相关性

Association between fear and endothelium diastolic dysfunction in spontaneous hypertension rats

目的探讨恐志因素与自发性高血压大鼠(SHR)血管内皮舒张功能之间的关系。方法选取20只SHR按简单随机抽样方法分为SHR对照组和SHR恐志组,每组各10只;另设WKY大鼠10只为正常对照组。参照Takashi方法,通过测定3组大鼠血浆一氧化氮(NO)、内皮素(ET)、血清可溶性细胞间黏附分子1(sICAM-1)、血管内皮细胞黏附分子1(VCAM-1)水平及观察胸主动脉壁内皮细胞的形态学变化,探讨恐志对SHR血管内皮舒张功能的影响。结果 SHR恐志组大鼠的冻结时间较SHR对照组明显延长[(10.93±1.38)s比(3.95±0.58)s,P<0.01];与SHR对照组比较,WKY组大鼠的ET、VCAM-1和sICAM-1水平较低[(54.700±4.607)ng/L比(66.213±5.420)ng/L;(780.190±96.015)ng/L比(942.742±123.127)ng/L;(0.340±0.138)μg/L比(0.794±0.243)μg/L],NO水平较高[(44.200±1.733)μmol/L比(35.450±1.435)μmol/L],均为P<0.01;与SHR对照组比较,SHR恐志组ET[(71.348±4.764)ng/L]和NO[(37.020±1.280)μmol/L]水平升高(均为P<0.05),VCAM-1[(1040.746±140.803)ng/L]和sICAM-1[(0.847±0.192)μg/L]水平略高(均为P>0.05);与WKY组比较,SHR恐志组ET、VCAM-1和sICAM-1水平升高,NO水平降低(均为P<0.01)。扫描电镜显示,3组大鼠胸主动脉内皮细胞的形态均不相同:WKY组为正常大鼠胸主动脉壁内皮细胞形态;SHR对照组轻者内皮细胞基本上仍呈梭形,细胞膜呈条状撕脱,重者细胞皱缩变小,细胞膜损伤明显,并可见内皮下层的裸露区,无沉积物;SHR恐志组大鼠内皮细胞排列极度紊乱,细胞损伤严重,脱落较彻底,内皮层表面沉积物较多,被粗细不等、交错成网的纤维状物质包裹。结论恐志可以影响SHR的血管内皮舒张功能。

Objective To investigate the association between emotional stimulation（fear） and endothelium diastolic dysfunction in spontaneous hypertension rats（SHR）. Methods Twenty SHR were randomly divided into Group B（SHR control group,n=10） and Group C（SHR with emotional stimulation of fear,n=10）.10 Wistar Kyoto rats as normal control group（Group A）.Plasma levels of nitric oxide,ET,sICAM-1 and VCAM-1 were measured,and the morphologic changes of endothelium of thoracic aorta wall were evaluated. Results The freezing time in group C was significantly longer than in group B [（10.93±1.38） s vs.（3.95±0.58）s,P0.01].The levels of plasma ET,VCAM-1,sICAM-1 were significantly lower [（54.700±4.607） ng/L vs（66.213±5.420） ng/L for ET;（780.190±96.015） ng/L vs（942.742±123.127） ng/L for VCAM-1 and（0.340±0.138） μg/L vs（0.794±0.243） μg/L for sICAM-1,all P0.01] and the level of NO was significantly higher[（44.200±1.733） μmol/L vs（35.450±1.435） μmol/L,P0.01] in group A than in group B.Plasma levels of ET,VCAM-1 and Sicam-1 were significantly increased and NO was significantly decreased in group B than in group C or in group A.Scanning electron microscopy showed that the endothelial cells in thoracic aorta are injured most severely in group C. Conclusions Conclusions Emotional stimulation of fear can affect the diastolic function of vascular endothelial cells in SHR.