摘要
目的 :研究人牙胚不同发育时期Calbindin -D2 8K(CaB)的表达 ,以期分析CaB在人牙体硬组织形成过程中的作用 ,为探明人牙齿矿化过程中经细胞钙转导机制提供实验依据。方法 :免疫组化ABC染色。结果 :人牙胚蕾、帽状期成釉器无CaB的表达 ;钟状早期成釉器的内釉细胞及部分牙乳头表面处于分化状态的细胞弱阳性染色 ;钟状晚期成釉器的成釉细胞、成牙本质细胞及部分胞核呈现强阳性染色。结论 :CaB参与了人釉质及牙本质的形成过程 ,并且在人牙胚发育过程中的CaB表达具有时间特异性及细胞特异性。
AIM: To investigate the express of calbindin-D28K(CaB) in human tooth germ development and its possible role in biomineralization during tooth development. METHODS: ABC immunohistochemical technique. RESULTS: CaB was not detected during early odontogenesis from the dental lamina to the late cap stage .Small amount of the protein first appeared in ameloblast and some of differenting cells in the surface of dental papilla in early bell stage. As to late bell stage, the cytoplasm of ameloblasts and odontoblasts with secreted dentin was all stained strongly, as well as some of nuclei. CONCLUSION: CaB participated in the human odontogenesis. Besides, the express of CaB in human tooth germ development was time-specific and cell-specific.
出处
《牙体牙髓牙周病学杂志》
CAS
2000年第2期63-66,共4页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金!(39870 789)
