丹红注射液对大鼠心肌缺血再灌注损伤后血清黏附分子1及炎症因子影响的实验研究

Experimental Study of Danhong Injection on Myocardial Inflammation by Ischemia-Reperfusion in Rats

目的:探讨丹红注射液对大鼠心肌缺血再灌注(I/R)后血清炎症因子的影响。方法:30只雄性SD大鼠随机分为假手术组(10只)、模型组(10只)、药物组(10只)。模型组和药物组大鼠通过穿线结扎左冠状动脉前降支与松开的方法制备心肌缺血再灌注损伤模型(心肌缺血30min,再灌注2h)。假手术组只穿线不结扎。药物组大鼠在模型建立前5天开始每天给予丹红注射液2mL/kg腹腔注射,假手术组和模型组只给予同等剂量的生理盐水腹腔注射。各组心肌缺血30min,再灌注2h采血离心取上清采用ELASA的方法检测血清中ICAM-1及白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、的水平。结果:与假手术组比较,模型组大鼠的IL-1β、IL-6、TNF-α、ICAM-1水平明显升高(P均<0.05);与模型组相比,药物组大鼠的IL-1β、IL-6、TNF-α、ICAM-1水平明显降低(P均<0.05)。结论:丹红注射液可以降低心肌缺血再灌注损伤的黏附分子1(ICAM-1)及炎症因子水平,具有心肌保护作用。

Objective：To study the effects of Danhong injection on myocardia ICAM-1 and inflammation by ischemia-reperfusion（I/R） in rats. Methods：Thirty healthy male Sprague Dawley rats were randomly divided into three groups：Sham-operated group（10） , Model group（10） and Drug group（10）. Myocardial ischemia reperfusion injury model was induced by the ligation of left descending anterior coronary artery （LAD） for 30min, followed by 120min of reperfusion in Model and Drug groups. From five days before surgery, Danhong injection （5mL/kg, per day, for Drug group）or saline （for Model and Sham-operated groups）was ad- ministered via peritoneal injection ;blood samples were collected from the carotid artery after 2h of reperfusion, to determine the levels of tumor necrosis factor-a （ TNF-a）, interleukin - 1β （ IL - 1β ）, interleukin - 6 （ IL - 6 ） and intercellular adhesion molecule - 1 （ ICAM - 1 ） using enzyme-linked immunosorbent assays. Results ： Compared with sham-operated group, levels of TNF - a, IL - 1β, IL - 6 and ICAM - 1 were significantly increased（ allP 〈 0. 05 ） ; compared with Model group, levels of TNF - a, IL - 1β, IL - 6, ICAM - 1 in the Drug group were decreased （allP 〈0. 05 ）. Conclusion：Danhong injection is effective in protecting the myocar- dium against MI/R injury in rats. Its possible cardioprotective mechanism involves inhibition of the inflammatory response follow- ing MI/R injury.