反相高效液相色谱法测定复方红景天心脑冲剂中红景天苷的含量

REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF SALIDROSICIE IN RHODIOLA XINNAOCHONGJI

目的 :建立测定复方红景天心脑冲剂中红景天苷含量的方法。方法 :采用反相高效液相色谱法 (RP HPLC)测定了红景天苷的含量。以C18化学键合硅胶为固定相 ,甲醇 水 (10∶10 0 )为流动相 ,紫外检测波长 2 78nm。结果 :方法的平均加标回收率为 98.84% (n =5 ) ,相对标准差RSD为 2 .2 % (n =5 ) ,红景天苷在 4～ 12 μg范围内 ,浓度与面积呈良好的线性关系 ,r =0 .9997。结论 :方法简便、快速 ,结果准确 ,重现性好 。

OBJECTIVE:To establish a method for determination of Salidrosicie in Rhodiola xinnaochongji. METHODS:The Salidrosicie content was determined by RP HPLC using methanol∶H 2O(10∶100)as mobile phase and a UV detector at 278nm.RESULTS:An average recoverage of 98.84% ( n =5)was obtained with a RSD of 2.2%( n =5)and a linear range of 4～12μg,r=0.9997.CONCLUSION:A simple,rapid method for determination of Salidrosicie in Rhodiola xinnaochongji is established with an excellent reproducibility.