变构菌素产生菌S.griseochromogenes接合转移体系建立与优化

Establishment and optimization for conjugal transfer system of tautomycetin-producing strain S.griseochromogenes

以整合型质粒pSET152为出发质粒,建立并优化了变构菌素产生菌Streptomyces griseochromogenes(S.griseochromogenes)与Escherichia coli(E.coli)的属间接合转移体系,确定了适用于变构菌素产生菌灰产色链霉菌的最佳接合转移条件.研究发现Ca2+和甘氨酸都能够提高接合转移的效率,尤其是甘氨酸的效果非常显著.这一工作对于S.griseochromogenes的基因操作、小分子药物生物合成机制的研究以及具有自主知识产权的药物衍生物的开发具有普遍的应用价值.

Intergeneric conjugal transfer system from Escherichia coli（E.coli） to tautomycetin-producing strain Streptomyces griseochromogenes（S.griseochromogenes） is established and optimized,which uses integrative plasmid pSET152 as starting plasmid.The best conjugal transfer conditions for S.griseochromogenes are demonstrated.And it is found that Ca2＋ and especially glycine,can increase the efficiency of exconjugates.The experimental results are very helpful to genetic manipulation of S.griseochromogenes,bio-synthesized mechanism research for small molecule drugs and for developing new analogs of drug with independent knowledge property.