TPM1基因在猪背最长肌生长发育过程中的动态表达

Dynamic Expression of TPM1 Gene During Longissimus dorsi Muscle Development in Pigs

【目的】探究原肌球蛋白(tropomyosin,TM)是否在猪背最长肌生长发育过程中发挥生物学功能。【方法】采用SYBR Green Ⅱ荧光法进行荧光定量PCR分析TPM1基因在通城猪和长白猪中的组织表达谱,以及出生前和出生后背最长肌发育中(28个发育点)的表达变化。【结果】TPM1基因在两种猪中背肌和心肌中都有丰富地表达,在背最长肌发育过程中的表达趋势相似,即TPM1基因的表达量先增加后减少,但出现峰值的时间不同(通城猪中,TPM1基因在胚胎期75 d的表达量显著高于其它时间点,P<0.05;长白猪中,TPM1基因在胚胎期90 d的表达量显著高于其它时间点,P<0.05)。【结论】TPM1基因的表达与猪背最长肌生长发育及品种间肌肉表型差异有关。

【Objective】The purpose of the present research was to explore whether tropomyosin（TM） has biological functions in the regulation of the Longissimus dorsi muscle growth and development of pigs.【Method】In this study,TPM1 gene expression profiles in different tissues from Tongcheng pigs and Landrace pigs were analyzed and the expression change during Longissimus dorsi muscle development（28 stages） was investigated by real-time qPCR with SYBR Green Ⅱ.【Result】The result showed that the TPM1 gene was strongly expressed in the Longissimus dorsi muscle and heart,and both breeds were similar in the expression patterns with decreasing after increasing,but the peak occurred at different times.The expression level of TPM1 gene in embryonic period 75 d were significantly higher than those in other periods in Tongcheng pigs,and the same result was found in embryonic period 90 d in Landrace pigs.【Conclusion】Results suggested that TPM1 gene expression may be related to the Longissimus dorsi muscle growth and muscle phenotypic differences between species of pigs.