盐酸胺碘酮的电化学测定及与牛血清白蛋白的相互作用

Electrochemical Determination of Amiodarone Hydrochloride and Its Interactions with Bovine Serum Albumin

建立了测定盐酸胺碘酮(Am)的新的电化学分析方法。采用电化学方法结合紫外、红外光谱分析,研究了Am与牛血清白蛋白(BSA)的相互作用。实验发现,在pH=5.0的B-R缓冲溶液中,Am在0.87V处有一灵敏的氧化峰,其氧化峰电流Ipa与Am的浓度在1.8×10-7～6.7×10-5 mol/L范围呈良好的线性关系,检出限(S/N=3)为6.0×10-8 mol/L。当BSA加入Am溶液后,Am的氧化峰电流降低,其氧化峰电流的降低值△Ipa与BSA的浓度在2.4×10-7～2.2×10-5 mol/L范围内呈良好线性关系,BSA的检出限(S/N=3)为9.0×10–8 mol/L。电化学研究表明,Am与BSA之间形成1∶1的结合物,结合常数为9.9×106 L/mol。紫外光谱表明Am的加入使BSA的吸收峰发生红移且有增色效应。傅立叶红外光谱表明Am与BSA分子中氨基酸残基的硫及氮原子形成键合作用。

A electrochemical method for the determination of amiodarone hydrochloride（Am）was developed.The interactions between Am and bovine serum albumin（BSA）were investigated by electrochemical method,UV absorption spectra and IR spectroscopy.In pH=5.0 Britton-Robinson buffer solution,an irreversible oxidation peak of Am was observed on the MWCNT/GCE at 0.87V.The anodic peak current was linear with the concentration of Am in the range of 1.8×10-7～6.7×10-5 mol/ L with a detection limit of 6.0×10-8 mol/L.In the presence of BSA,the oxidation current of Am was decreased and the decreasing value was linear with the BSA concentration in the range of 2.4×10-7～ 2.2×10-5 mol/L,with a detection limit of 9.0×10-8 mol/L.The binding equilibrium constant and binding ratio was calculated to be 9.9×106L/mol and 1∶1.The results of UV absorption spectra showed that the UV absorption peak of BSA could be red shifted and the maximum absorption value was increased.The results of FTIR spectroscopy showed that Am could interact with sulfur-containing and nitrogen-containing groups in BSA molecules.