白藜芦醇与DNA相互作用的研究

Studies on the Interaction of Resveratrol with Deoxyribonucleic Acid

在pH=7.4的Tris-HCI缓冲溶液中,采用紫外光谱、荧光光谱及粘度法研究了白藜芦醇与小牛胸腺DNA(ctDNA)的相互作用。在生理条件下,白藜芦醇可以与ctDNA相互作用形成复合物,运用双倒数法求出了白藜芦醇与ctDNA相互作用的热力学参数。白藜芦醇能有效猝灭吖啶橙(AO)-DNA体系的荧光,猝灭方式为生成复合物的静态猝灭。粘度法实验证明ctDNA溶液的粘度随着白藜芦醇浓度的增加而增大。结果表明,白藜芦醇与DNA发生了较强的相互作用,其键合模式为经典的插入方式。

The interaction of resveratrol with calf thymus deoxyribonucleic acid（ctDNA）under physiological conditions（Tris-HCl buffer solutions,pH =7.4）was studied by UV spectroscopy,fluorescence spectroscopy and viscosity measurements.Results indicate that a complex of resveratrol and ctDNA was formed,and the thermodynamic parameters of the complex were calculated by a double reciprocal method.The fluorescence quenching mechanism of AO-ctDNA by using resveratrol was a static quenching type.By combination of viscosity determination results,we suggested that the interaction model between resveratrol and ctDNA be the intercalation bindings.