精氨酸与2,3-二氯-5,6-二氰-1,4-苯醌的荷移反应研究

Study on Charge Transfer Reaction of L-Arginine with 2,3-Dichloro-5,6-chicyano-1,4-benzoquinone

用分光光度法研究了精氨酸与2,3-二氯-5,6-二氰-1,4-苯醌(DDQ)的荷移反应。实验表明,在硼砂溶液中,精氢酸与DDQ在室温下反应30min可获得稳定的络合物,其组成比为2∶1,λmax=342nm,表观摩尔吸光系数ε=1.65×104 L.mol-1.cm-1,线性范围为2～30μg/mL。应用拟定的方法测定了盐酸精氨酸注射液的含量,结果与文献方法一致,回收率在97.2%～98.1%之间,相对标准偏差小于3.1%。

A simple,quick and sensitive spectrophotometric method was established for the determination of L-arginine,based on charge transfer reaction with 2,3-dichloro-5,6-chicyano-1,4-benzoquinone（DDQ） in borax buffer solution.The apparent molar absorptivity of the complex was determined as 1.65×104L.mol-1.cm-1 at 342nm.The calibration curve was linear in the concentration rang of 2～30 μg/mL.The composition of complex of L-arginine with DDQ was found to be 2∶1by Job’s method.The proposed method has been applied to the determination of L-arginine in injections with satisfactory results.