大黄的生化学研究ⅩⅩⅪ.波叶大黄多糖对免疫功能的促进作用

Biochemical Study of Chinese Rhubarb ⅩⅩⅪ. Effect of Polysaccharide From Rheum hotaoense on Immune System

从波叶大黄中提取、分离得到波叶大黄多糖(Rheum hotaoense polysaccharide, RHP),ig RHP 100和200 mg/kg能明显增加小鼠脾脏和胸腺重量,与对照组相比,脾指数分别增加30%和38%,200 mg/kg的剂量使胸腺指数增加25%;能促进小鼠腹腔巨噬细胞吞噬功能,吞噬百分数分别比对照组增加38%和61%。200 mg/kg的剂量可使吞噬指数增加155%;能促进小鼠碳粒廓清速率,K值分别比对照组增加48%和217%;能促进SRBC致敏小鼠血清溶血素形成,HC_(50)分别比对照组增加11%和57%;能促进小鼠抗体形成细胞的生成,QHS分别比对照组增加63%和126%。RHP还可明显促进受ConA诱导的小鼠脾淋巴细胞DNA的合成,最适浓度为20μg/ml,刺激指数为1.61;对ConA活化的脾淋巴细胞蛋白质合成也有明显促进作用,刺激指数为1.56;对ConA诱导的淋巴细胞IL-2产生有明显增强作用。

The polysaccharide from Chinese Rheum hotaoense (RHP) was isolated and its effects on immune system were studied. The results showed, at the doses of 100 and 200 ms/kg by is administration for 7 days in mice, RHP increased the thymus and spleen weights; the phagocytosis of macrophage, the clearance rate of carbon particles, the production of hemolysins, and the response of lymphoid cells immune to sheep red cells (QHS). RHP also showed a remarkable effect on DNA, protein synthesis and IL-2 production of mice spleen lymphocytes with ConA stimulation. The optimal concentration of RHP for increasing DNA synthesis was 20 μg / ml. The stimulation index was 1.61 for DNA synthesis and 1.56 for protein synthesis. It is suggested that RHP has the effect on immune functions and the effect is quite opposed to those of anthraquinone derivatives of Rheum. Therefore, we suggest that there are some components with the different effects in the Rheum on the immune system.