液相色谱流出峰定量法研究Ⅰ.从检测峰求算流出峰

Study on Elution Peak Quantitative Method for HPLC Ⅰ. Calculate Eluting Curve from Detecting Peak

液相色谱包括管内流动、柱上分离和池内检测三个过程。本文研究讨论了检测过程对色谱峰的影响,提出了由检测峰求算流出峰的保留时间,峰面积、峰高和峰宽的方法并由实验证实,从而消除了检测过程的影响,得出有可比性的规范化的流出峰,这有利于理论研究并建立了一种新的省时省事、更为准确的液相色谱流出峰定量法。本文还讨论和给出校正流动影响的实验方法。首次提出峰锐度概念以表征峰的顶部特征。

Thcre are three processes in HPLC, i. e. flow in tube, separation in column and detection in thru-cell, the influence of detecting process on chromatographic peak is studied and the calculating formulae of retention time, peak area, peak width and peak high of eluting curve from detecting peak are presented, those formulae have been confirmed by experiments, the effect of detailed detecting processes can be eliminated and the standardized eluting curve, which is advantageous to theoretical study, can be obtained. Thus a novel quantitative method—eluting curve method—can be established. The method is time-saving, simpler and more precise. In this paper, the experimental method for correcting the influence of flow process is suggested and a new concept of SHARPNESS is first suggested to characterize the feature of the top of chromatographic peak.