利用离子交换树脂制备弹性酶

Preparation of Elastase by Ion Exchange Resin

采用SP树脂制备弹性酶。每公斤猪胰脏可得比活为30.69 u/mg的弹性酶7.03g。活力回收率为66.0%。同时还将SP树脂与国内生产弹性酶的J_2树脂、113树脂及Amberlite CG50进行了比较。实验结果表明:利用SP树脂吸附弹性酶后的母液仍可回收胰激肽释放酶。

Elastase was adsorbed by SP resin. Elution was carried out by NH_4Ac and precipitation by aceton. The average yield and activity of elastase were 7.03 g/kg pancrease and 30.69 u/mg, respectively. The recovery rate was 66.0%. The average yield and enzyme activity extracted by SP resin were compared with those by 113 resin, J_2 resin and Amberlite CG50. The experiment showed that kallikrein in the mother solution in which elastase had been removed by SP resin could be prepared.