金钗石斛的离体快繁体系研究

Study on the in vitro Rapid Propagation System of Dendrobium nobile Lindl.

[目的]研究金钗石斛的离体快繁体系。[方法]以金钗石斛的种子为材料,采用组培法进行离体培养。[结果]适宜于金钗石斛圆球茎诱导增殖及芽分化的培养基为:MS+1 mg/L BA+0.02 mg/L NAA+浓度2%马铃薯+浓度3%白糖,增殖系数为7～10。生根培养基为1/2MS+0.2 mg/L BA+0.2 mg/L NAA+0.2 mg/L GA+浓度2%香蕉+浓度2%马铃薯+浓度2%白糖;生根率达98%以上,移栽成活率在90%左右。[结论]该方法对金钗石斛的离体快繁体系进行了研究,为组培繁殖金钗石斛种苗提供了参考。

[ Objective ] To study the in vitro rapid propagation system of D. nobile. [ Method ] The seeds of D. nobile were selected for the in vitro rapid propagation by tissue culture. [Result] MS ＋ 1 mg/L BA ＋ 0.02 mg/L NAA ＋ 2% potato juice ＋3 % sucrose was suitable for the protocorm induction and bud differentiation of D. nobile. The inductive rate was 7 - 10. The most suitable rooting medium was 1/2 MS ＋ 0.2 mg/L BA ＋ 0.2 mg/L NAA ＋ 0.2 mg/L GA ＋ 2% banana juice ＋ 2% potato juice ＋2% sucrose. The rooting rate was above 98% , and survival rate was above 90%. [ Conclusion] The method studied the in vitro rapid propagation system of D. nobile and provided references for the tissue culture of its seedlings.