金银花超微粉碎前后绿原酸溶出量和细粉显微特征的比较

Comparation of Dissolution Content of Chlorogenic Acid and Microscopic Characteristics of FLOS LONICERAE JAPONICAE Before and After Ultramicro Pulverizing

[目的]研究超微粉碎前后金银花中绿原酸溶出量的变化及显微特征。[方法]用不同时间超声提取超微粉与普通粉,并用HPLC法测定绿原酸含量;然后采用生物显微镜进行显微特征观察。[结果]金银花超微粉碎后绿原酸的溶出量和溶出速率明显提高;金银花超微粉颗粒均匀,基本无完整细胞存在。[结论]超微粉碎有助于金银花中绿原酸的溶出,植物细胞破壁率明显提高。

[ Objective ] To compare the dissolution content of chlorogenic acid and microscopic characteristics of FLOS LONICERAE JAPONICAE before and after ultramicro pulverizing. [ Method] The content of chlorogenic acid in the ultramicro powder and common power of FLOS LONICERAE JAPONICAE prepared with different times was determined by HPLC. Microscopic characteristics of the two sorts of powder were surveyed by biological microscope. [ Result] The dissolution content and dissolution rate of chlorogenic acid in the powder increased significantly after ultramicro pulverizing. Compared with common powder, the ultramicro powder particles were more uniform in shapes and nearly no completed cells were observed. [ Conclusion ] Ultramicro pulverizing procedure can benefit the dissolution content of ehlorogenic acid and significantly improve the wall-broken rate of plant cells.