应用特异PCR方法诊断2例猪弓形虫病

Diagnosis of Swine Toxoplasmosis by PCR Assay

[目的]应用特异PCR方法诊断猪弓形虫病。[方法]分别以弓形虫核糖体DNA第一内部转录间隔区(ITS1)序列和529 bp重复序列为目的片段,扩增2例疑似猪弓形虫病的病料肺门淋巴结DNA,对疑似猪弓形虫病的病料进行PCR诊断。[结果]2对引物均能分别扩增出2例肺门淋巴结中弓形虫DNA的特异条带。[结论]基于2种扩增片段的PCR方法均具有较高的特异性,是诊断猪弓形虫病的一种快速检测方法。

[ Objective ] The aim was to diagnose swine toxoplasmosis by PCR assay. [ Method ] Two cases of suspected swine toxoplasmosis from different farms were collected by clinical diagnosis. The hilar lymph node DNA of the two cases were extracted and the first internal transcribed spacer of ribosomal DNA （ ITS1 ） and the repetitive 529 bp fragment were amplified by PCR assay using two pairs of primers, respectively. [ Result] The DNA fragments specific for Toxoplasma gondii were both amplified in ITS1 and the repetitive 529 bp fragment from infected tissues genomic DNA in both cases by PCR assay, respectively. [ Conclusion] Based on two genetic markers, PCR assay is a rapid and accurate method for diagnosing swine toxoplasmosis with high specificity.