铁·镁·钙元素对白腐真菌生长及降解石油的影响

Effect of Fe^(2+),Mg^(2+) and Ca^(2+) on Oil Degradation and the Growth of White Rot Fungi

[目的]探讨黄孢原毛平革菌的生长及对石油的降解能力的优化条件。[方法]选用白腐真菌的典型菌种——黄孢原毛平革菌作为降解菌,研究其生长及对石油的降解性能,以不同浓度的Fe、Mg、Ca元素作为研究对象,在筛选单因素最佳水平的基础上,设计L9(33)正交表进行正交试验,对各因素的重要性及其最优水平进行分析。[结果]单因素试验表明,体系中添加Fe、Mg、Ca元素对白腐真菌的生长及其对石油的降解能力有较大的影响。正交试验结果表明,3种元素对P.C.菌降解石油的影响大小依次为Fe2+>Ca2+>Mg2+;溶液中铁、镁、钙元素的最佳组合为0.008 g/L的Fe2+、0.8 g/L的Mg2+、1.5 g/L的Ca2+,该条件下10 d后白腐真菌对石油的降解率为70.9%。[结论]该研究为探索白腐真菌的生长及其对石油降解能力的优化提供了科学依据。

[ Objective ] The study aimed to discuss the growth and degradation of petroleum by white rot fungi. [ Method ] Taking the typical white rot fungi P. chrysosporium as the degrading fnngus,its growth and degradation on the petroleum were studied. The effect of cations including Fe2＋ ,Mg2＋ and Ca2＋ were selected as the effecting factors,on the basis of screening the best level of single factor,the orthogonal experiment with the L L9 （33） orthogonal design was conducted to analyze the importance of the factors and their optimal level. [ Result] The single test showed that Fe2＋ ,Mg2＋ and Ca2＋ had a greater influence on the growth of white rot fungi, and biodegradation of petroleum. The results of orthogonal experiment showed that Fe2＋ , Mg2＋ and Ca2＋ had significant influence on the degradation of petroleum by white rot fungus,the effect was Fe2＋ 〉 Ca2 ＋ 〉 Mg2 ＋ , the Degradation can reached up to 70.9% under the optimum conditions： [ Fe2 ＋ ] = 0. 008 g/L, [ Mg2 ＋ ] =0.8 g/L, [ Ca2＋ ] = 1.5 g/L. [ Conclusion] The study provided the scientific basis for exploring the effective method of degrading the petroleum and the growth of white rot fungi.