高效液相色谱-紫外光检测法测定食品中罗丹明B的含量

Determination of Rhodamine B in Food by HPLC-UV Method

[目的]建立食品中罗丹明B的高效液相色谱-紫外光检测法。[方法]将样品中的罗丹明B用丙酮、正己烷提取,经过氧化铝固相萃取柱净化浓缩后,利用高效液相色谱紫外可见光检测器检测其含量。[结果]罗丹明B在0.005～2.000 mg/kg浓度范围内,峰面积与浓度呈良好线性关系,相关系数为0.999 98;方法的定量检测限为0.005 mg/kg,且平均回收率高。[结论]该方法适用于食品中罗丹明B的测定,并具有快速、准确、灵敏度高的优点。

[ Objective ] The aim was to establish a method for the determination of Rhodamine B in food by HPLC-UV. [ Method ] Rhodamine B was extracted with aeetone/hexane from food samples. After concentrated and purified by alumina cartridge,the Rhodamine B content in the food was determined by using high performance liquid chromatography with ultraviolet visible detector. [ Result] Within the concentration range of 0. 005 -2. 000 mg/kg, the peak area of Rhodamine B presents good linear relation with the concentration with the related coefficient of 0. 999 98. The detection limit of the method is 0. 005 mg/kg,and with high average recovery rate. [ Conclusion] It is a fast and accurate method to detect Rhodamine B in food with high sensitivity.