六味地黄煎剂对心、肾、脑缺血的实验治疗

Experimental Treatment of Ischemic Injury in the Heart, Kidney and Brain in Rats by the Chinese Traditional Prescription Referred as Liuwei Dihuang Decoction

六味地黄煎剂(LD)po 5g/kg,qd×3d,能明显对抗大鼠由于左冠脉结扎而引起的心肌中超氧化物歧化酶(SOD)活性的降低及丙二醛(MDA)含量的增加。提示它能明显抑制梗塞心脏中氧自由基的大量生成。形态学指标亦证实,LD po 5g/kg,qd×5d,能明显缩小心肌缺血再灌大鼠的梗塞区面积,同时增加灌流区面积。LD对大鼠肾缺血有一定的保护作用,而对大鼠脑缺血及小鼠肾、脑缺血均无明显作用。

Liuwei Dihuang Decoction (LD) administered orally 5g/kg for 3 d showed beneficial effect on protection of SOD activity and inhibition of MDA production when rats were suffered from left coronary artery ligation for 15 min. In another isehemia model of ligating the left coronary artery for 1 h then reperfusion for 24 h in rats, we also observed morphologically that LD reduced the infarcted myocardium significantly. The prescription was able to increase SOD activity in the globally ischemic kidney, but no protection on cerebral ischemia was noticed. This study offers an evidence for clinical application of the traditional prescription to the patients suffering from coronary disseases or acute/chronic myocardial infarction.