自体树突细胞与混合T淋巴细胞疫苗治疗慢性乙型肝炎的护理

Nursing effect on blood collection and doping for chronic hepatitis B patients treated with self dendritic cells and mixed T lymphocytes vaccine

目的探讨乙肝患者使用自体树突细胞和混合T淋巴细胞疫苗（简称抗HBV—DC-MTL）治疗慢性乙型肝炎的临床采血与回输的护理。方法取乙肝患者的肝素抗凝外周静脉血50ml，通过实验室生物免疫技术培养获得成熟的树突细胞和混合T淋巴细胞疫苗，培养第7天将收获的DC、第14天收获的抗HBV-MTL分别回输给患者。并对38例乙肝患者采集细胞与回输的护理进行观察。结果38例患者好转36例，无效1例，治愈1例。结论采用肝素抗凝的50ml注射器加9号输液头皮针在大血管处采血，采血成功率高；标本4℃左右冷藏保存，2h内分离培养，是收获细胞疫苗成功的关键；回输的方法，安全度高，并发症少。

Objective To explore theclinical blood collection and doping nursing care for hepatitis B patients who use self dendritic cells （DC）and mixed T lymphocytes vaccine （resistant HBV-DC-MTL for short） for treatment. Methods Thirty-eight chronic hepatitis B virus （HBV） infection patients including 33 chronic hepatitis B and 5 chronic HBV carriers undergone clinical trial during December 2010 to July 2011. Fifty millilitre heparin anticoagulation peripheral venous blood was taken for cultivating mature DC and mixed T lymphocytes vaccine with laboratory biological immune technology, the DC obtained on the seventh day and resistant HBV-MTL obtained on the fourteenth day intravenously injected to the patients and we observed the cell collection and doping nursing effect. Results No case was recollected blood for blood coagulation, the obtained cells after cultivated were returned to the patient without adverse effects and showed a distinct effect in 38 patients. Conclusions It is more successful in blood collection by using the heparin 50 ml syringe and anticoagulant 9 infusion scalp needle in the great vessels; it is the key in obtaining cell vaccine that the specimen is cold preservation in 4 ℃, and separated and cultured in 2 h; doping method is of high safety and less complications.