19种氨基酸的反相高效液相色谱分析

Assay of 19 Amino Acids by Reversed-phase High Perfermannce Liquid Chromatography

本文报道了一种快速、高灵敏度的邻苯二甲醛-氨基酸衍生物的高效液相色谱分析法。本法可在27min内完成工9种氨基酸的分离测定;最低检出量<1 pmol;各氨基酸的峰面积-浓度线性范围为1.5～150μml/L,r=0.999;测定精密度(C、V范围):保留时间为0.1～0.9％,峰面积为0.6～5.4％(蛋氨酸、酪氨酸除外),回收率为113.5～72.2％。本文还讨论了pH,流动相配比、不同梯度形式对氨基酸分离的影响。

This paper reports a rapid and high sensitive method of analysing O-pht haldialdehyde-amino acids with HPLC. A mixture of 19 amino acids fluoresc- ent derivatives could be resolved within 27 minutes. The decective limit for individual amino acids is below 1 pmol. The peak area-concentration linear range for individual amino acids was 1.5-150 umol/L, r=0.999. The precis- ion (C.V) studies for TR and peak area provided 0.1-0.9% and 0.6-5.4% (except Met and Tyr) separately. The average recoveries was 113.5-72.5%. The effects of pH, mobile phase, and elution gradient for separation of ami- no acids are discussed.