头针对脑缺血再灌注大鼠脑微血管Ⅳ型胶原蛋白影响的动态研究

Dynamic Study of Scalp Acupuncture on Regulation of Expression of Type Ⅳ Collagen in Rats with Middle Cerebral Artery Occlusion

目的:观察头针对脑缺血再灌注大鼠脑微血管Ⅳ型胶原蛋白的动态影响,以探讨头针抗脑缺血再灌注损伤的可能作用机制。方法:SD大鼠130只,随机分为假手术组、模型组和针刺组。采用改良的Zea Longa线栓法复制脑缺血再灌注模型,应用免疫组化和ELISA测定,比较各组在不同时点脑微血管基底膜Ⅳ型胶原蛋白阳性表达与含量的变化。结果:头针组大鼠再灌注4 h、12 h,梗死灶周围区的Ⅳ型胶原阳性表达及含量均与模型组相似(P>0.05);再灌注24 h、48 h、72 h较模型组有所增加(P<0.05),但仍低于假手术组(P<0.05)。结论:早期头针治疗能上调缺血侧脑组织中Ⅳ型胶原蛋白的表达,并随着针刺治疗时间的延长,其效果明显提高。通过上调Ⅳ型胶原蛋白的表达,有助于保护血脑屏障,可能是头针拮抗脑缺血再灌注损伤的作用途径之一。

Objective： To investigate the effects of scalp acupuncture on collagen IV expression in the rats with cerebral ischemia reperfusion for exploring the interaction mechanisms. Methods： 130 Male SD rats were ran- domly divided into three groups：sham operation group, model group and scalp acupuncture group. Cerebral is- chemia/reperfusion model was induced by modified Longa＇ s method. We detected the positive expression and protein content of ColIV by immuno histochemistry and ELISA methods respectively in brain tissue of each set. Results：In acupuncturing scalp group, tile expression of ColIV about infarction was similar to the model group in IRgh and IR12 h after reperfusion （ P 〉 0. 05 ）, but was higher obviously than that in model group in IR24 h, IR48h and IR72 h after reperfusion （P 〈 0. 05 ）. And at the same phase point, the content of ColIV was still lower than that in the sham group （ P 〈 0. 05 ）. Conclusion： Scalp - acupuncture therapy can promote the ex- pression of ColIV in rats after acute cerebral ischemia/reperfusion, and there is a further increase with the treat- ment time prolonging. Scalp - acupuncture therapy can ameliorate cerebral ischemia reperfusion injury by possi- bly enhancing CollV expression and protecting blood -brain barrier.