结缔组织生长因子基因的克隆

Investigation of Connective Tissue Growth Factor's Gene Cloning

目的 克隆细胞即刻早期基因反应产物人结缔组织生长因子 (CTGF)基因 ,对分析细胞基因反应及用于创伤修复研究的理论和应用研究均具有较大意义。方法 使用组织培养的方法 ,建立了人脐静脉内皮细胞系 ,在适当刺激后 ,提取 m RNA,应用 RT- PCR方法 ,分段克隆人结缔组织生长因子基因 ,并经无义突变形成酶切位点使之连接。结果 经序列测定 ,发现该 CTGF基因具有完整编码区及不同于文献报道的 3′端非编码区结构的结缔组织生长因子基因。结论 获得一个新的人 CTGF基因。

Objective The human connective tissue growth factor(CTGF),as the responsive product of immediate early gene,was cloned for the investigation of cellular primary gene responsed and healing repairing. Methods RT PCR was performed with the mRNA of HUVE cell stimulated,the three clones of CTGF,via mutational modified,were ligated to a complete gene that identified with sequencing. Results The sequencing indicated that this CTGF gene contains complete coding region and the 3' noncoding region which is difference with the reported sequence. Conclusion A new human CTGF gene was cloned.