猴免疫缺陷病毒感染猕猴快速进展型死亡特征

Characteristic Immunodeficiency Syndrome of Rapid Fatal Type of Simian Immunodeficiency Virus Infected Monkeys

目的 观察猴免疫缺陷病毒 (simian imm unodeficiency virus,SIV ) SIVmac和 SIVmac2 5 1毒株感染猕猴快速进展型死亡的感染特征。方法 用 SIVm ac和 SIVmac2 5 1静脉接种 ,实验性感染 80只恒河猴和 4只食蟹猴 ,定期采集静脉血浆进行病毒分离测定血浆病毒水平 ,间接免疫荧光法测定血浆病毒抗体水平 ,并取淋巴组织进行常规病理组织学检查。结果 84只猕猴感染 SIVmac和 SIVmac2 5 13～ 4个月 ,快速进展型死亡 12只 ,占14.2 %。快速进展型猕猴有高而持续的病毒血症 ,约半数有低抗体水平的特征。 10只快速进展型猕猴的病理组织学检查结果显示 ,淋巴结和脾脏呈现淋巴组织耗竭者占 80 %～ 90 % ,表明快速进展型死亡猴免疫功能已极度低下 ,80 %有合并症发生 ,其中以肠炎为主 ,主要死因为严重免疫衰竭或机会性感染。结论 SIV实验感染猕猴快速进展型死亡猴的特征为高而持续的病毒血症、约半数有低病毒抗体水平和严重的免疫功能受损。

Objective To observe the characteristic immunodeficiency syndrome of the rapid fatal type of simian immunodeficieny virus (SIV) infected monkeys. Methods Eighty rhesus monkeys and 4 cynomolgus monkeys were intravenously inoculated with SIVmac or SIVmac251. The virus isolation and viral titer, estimation by indirect immunofluroresence and viral antibody were determined periodily from monkeys' plasma; lymph node biopsies were performed for pathohistological examination. Results Twelve out of 84 macaque (14 2%) died of rapid progressive type after inoculation of SIVmac and SIVmac251 in the duration 3 to 4 months. Dying monkeys showed persistent high viremia and low level titre antibody. Eight of 10 pathohistological changes showed severe depletion of lymphiod tissue in spleen and lymph nodes, there were remarkable immunodeficiency with opportunity infection. The other two monkeys appeared moderate lymphoid tissue deletion and hyperplasia without opportunity infections. The survived monkeys' (72/84) lymph nodes biopsies revealed hypoplasia of lymphoid tissue. Conclusions The characteristic immunodeficiency syndrom of rapid fatal type of simian immunodeficiency virus infected monkeys could be made with persistent high viremia, low level antibody, severe lymphoid tissue deletion in lymph nodes and spleen, as well as complicated opportunity infections.