蛋白芯片技术联合检测抗dsDNA抗体、抗Smith抗体和抗Rib—P抗体对SLE诊断价值分析

Diagnostic Value Analysis about Anti-dsDNA Antibody, Anti-Smith Antibody and Anti-Rib-P Antibody of Systemic Lupus Erythematosus by Protein Chip Technology Combined Detection

目的评价蛋白芯片技术联合栓l刹抗dsDNA抗体、抗Smith抗体和抗Rib-P抗体对于系统性红斑狼疮（SLE）诊断的价值。方法采用蛋白芯片技术对85例SLE患者、129例其他自身免疫性疾病患者和43例健康体检者血清中的抗dsI）NA抗体、抗Smith抗体和抗Rib-P抗体进行检l蒯。结果SLE患者组血清中的抗dsDNA抗体、抗Smith抗体和抗Rib-P抗体的阳性率（50．59％，32．94％和37．65％）及其联合检测的阳性率（78．82％）明显高于疾病对照组（0．75％，2．33％，2．33％和5．43％），差异有统计学意义（，分别为100．269，52．36，62．481和155．51，P均〈O．01）。正常对照组均为阴性。SI．E患者抗dsDNA抗体的敏感度50．59％，特异度99．22％；抗Smith抗体敏感度32．94％，特异度97．67％；抗RibP抗体的敏感度37．65％，特异度97．67％；三项自身抗体联合检测的敏感度78．82％，特异度94．57％。联合检测的敏感度明显高于抗dsDNA抗体、抗Smith抗体和抗Rib-P抗体的单项检测。结论抗dsDNA抗体、抗Smith抗体、抗Rib-P抗体作为SLE重要的抗体，具有很高的特异度，在一张蛋白芯片上同时检测可互相补充，提高了SLE的诊断敏感度和诊断效率，减少了漏诊和误诊，对SLE的诊断、治疗和预后具有重要的意义。

Objective To evaluate the combined detection of the anti-dsDNA antibody,anti-Smith antibody and anti-Rib-P an- tibody in protein chip technique for the diagnosis of systemic lupus erythematosus （SLE）. Methods Detected the anti-ds DNA antibody, anti-Smith antibody and anti-Rib P antibody in the serum of 85 SLE patients, 129 patients with other autoim- mune diseases and 43 healthy subjects by applying protein-chip technology. Results The positive rate（50. 59% ,32. 94% and 37.65 %） of antbdsDNA antibody, anti-Smith antibody and anti-Rib-P antibody in SIzE patients′serum and the positive rate （78. 82%） of their combined detection was significantly higher than that of the disease control group. There was difference （χ^2：100. 269,52.36,62. 481 and 155.51, P〈0.01）. The normal control group was all negative. Among SLE patients,for the anti-dsDNA antibody,the sensitivity was 50. 59% and specificity was 99.22% ,for the anti-Smith antibody, the sensitivity was 32.94%and specificity was 97.67%; for the anti-Rib-P antibody, the sensitivity was 37.65% and specificity was 97.67% ;for the three autoantibodies＂ combined detection, the sensitivity was 78. 82% and specificity was 94. 57%. The sen- sitivity of combined detection was significantly higher than that of every single detection. Conclusion As the important SLE antibodies,anti-dsDNA antibody, anti-Smith antibody and anti-Rib-P antibody with high specificity can complement with each other in the simultaneous detection on a protein chip. It improve the sensitivity and efficiency of SLE diagnosis, reduce the missed diagnosis and misdiagnosis of SLE diagnosis, so it is important for SLE diagnosis, treatment and prognosis.