巨噬细胞移动抑制因子与细胞因子在原因不明早期复发性流产者血清和蜕膜中的表达及意义

Expression of macrophage migration inhibitory factor and Th1/Th2 cytokines in serum and deciduas in women with unexplained early recurrent spontaneous abortion

目的 研究巨噬细胞移动抑制因子（MIF）及Th1/Th2型细胞因子在原因不明早期复发性流产（UERSA）患者血清和蜕膜中的表达及意义.方法 选取因原因不明早期复发性流产患者22例（UERSA组）及同孕龄正常早孕妇女25例（正常早孕组）,检测两组外周血清中和蜕膜中MIF、IL-2和IL-10的浓度.并分析血清和蜕膜组织中MIF水平与IL-2水平的相关性.结果 两组血清和蜕膜中均有MIF和IL-2、IL-10的表达.UERSA组血清、蜕膜组织中MIF、IL-2、IL-10分别为（23.4±6.9）mg/L、（65.6±20.4）ng/L、（73.5±32.1）ng/L和（20.6±6.1）mg/L、（42.6±17.6）ng/L、（47.6±17.1）ng/L,正常早孕组分别为（38.4±9.8）mg/L、（39.3±12.4） ng/L、（93.4±25.4）ng/L和（33.7±8.6）mg/L、（27.2±9.5）ng/L、（64.7±28.2）ng/L;血清和蜕膜中MIF和IL-10 UERSA组均低于正常早孕组;IL-2水平则明显高于正常早孕组,差异有统计学意义;血清和蜕膜组织中MIF水平与IL-2水平呈负相关（r=-0.78、-0.65）.结论 妊娠期母胎界面MIF表达降低和Th1/Th2型细胞因子比率失衡,可能在原因不明早期复发性流产的发生发展中起重要作用.

Objective To explore the expression of macrophage migration inhibitory factor （MIF） and Th1/Th2 cytokines in serum and deeiduas in women with unexplained early recurrent spontaneous abortion （UERSA）. Methods 22 women with unexplained early recurrent spontaneous abortion （ UERSA group） and 25 women with normal early pregnancy （ normal pregnant group） were recruited from Heze Municipal Hospital between August 2010 to May 2011. Th1/Th2 cytokines were represented by IL-2/IL-10. Levels of MIF, IL-2, IL-10 in serum and deciduas were measured by enzyme linked immunosorbent assay （ ELISA）. The relationship between the levels of MIF and IL-2 in serum and dociduas were analyzed in the UERSA group and normal pregnant group. Results MIF, IL-2 and IL-10 were expressed in serum and deciduas in the UERSA group and the normal pregnant group. In the UERSA group, the expression of MIF, IL-2, IL- 10 N/S was （23.4 ± 6.9） mg/L, （65.6 ± 20.4） ng/L, and （73.5 ±32.1 ） ng/L, respectively, in serum and （20.6±6.1 ） mg/L, （42.6±17.6） ng/L, and （47.6±17.1 ） ng/L, respectively, in deeiduas, while in the normal pregnant group, they were （38.4±9.8） mg/L, （39.3 ±12.4） ng/L, and （93.4 ±25.4） ng/L, respectively, in serum and （33.7 ±8.6） mg/L, （27.2 ±9.5） ng/L, and （64.7 ±28.2） rig/L, respectively, in deeiduas. Serum and decidua MIF and IL-10 levels were markedly lower in the UERSA group than those in the normal pregnant group, while IL-2 levels were significantly higher in the UERSA group. There was a negative correlation between MIF and IL-2 in serum （ r = -0.78 ） and in meeiduas （ r = -0.65 ）. Conclusion Lower levels of MIF and the imbalance of Th1/Th2 in serum and deeiduas may contribute to the occurrence of unexplained early recurrent spontaneous abortion.