沙棘总黄酮对活性氧自由基的清除作用

SCAVENGING EFFECT OF TOTAL FLAVONOIDS OF HIPPOPHAE ON ACTIVE OXYGEN RADICALS

用ESR自旋捕集技术研究了沙棘总黄酮(TFH)对活性氧自由基(AOR)的清除作用,并用化学发光(CL)法测定了该药对多形核白细胞(PMN)产生CL的影响。结果表明1.7mg/L TFH对PMA刺激PMN生成的AOR有明显清除作用;TFH(0.03～3mg/L)对黄嘌呤/黄嘌呤氧化酶系统生成的O^(2·)有显著剂量依赖性的清除作用。该药对光照核黄素产生O^(2·)的清除作用较弱。TFH(3mg/L)对Fenton反应生成的OH·有明显清除作用。TFH(1mg/L)显著抑制PMN产生的CL。该药对O^(2·)的清除作用明显强于VitE。

The scavenging effects of Total Flavonoids of Hippophae ( TFH ) were studied by using ESR-spin trapping technique in different systems, and the inhibitery effects on chemiluminescence ( CL ) of poly-morphonuclear leukocytes ( PMN) were measured by using luminol-de-pendent CL. TFH ( 1.7mg/L ) significantly reduced the active oxygen radicals level of PMA-stimulated PMN; TFH ( 0.03-3mg/L ) could remarkably scavenged O2 in xanthine/xanthine oxidase (Xan/XO)system and the effect was concentration-dependent. The scavenging effect of TFH on O2 in irradiation riboflavin system was not so strong as in Xan/XO system. TFH ( 3 mg/L ) could scavenged OH produced in Fenton's reaction; TFH ( 1 mg/L ) effectively inhibited PMN CL stimulated by PMA.The effects of TFH on active oxygen radicals produced by PMN and O2 in Xan/XO system were stronger than Vit E.