Chk1/2蛋白在胶质瘤和正常脑组织中的分布及意义

Study on expression of Chk1 and Chk2 in glioma and brain tissue and their clinical significane

目的探讨Chk1、Chk2蛋白在胶质瘤和正常对照组织中的分布特点,以评价Chk1、Chk2作为胶质瘤治疗靶点的可行性。方法应用免疫组织化学SABC法检测Chk1、Chk2蛋白在80例胶质瘤和18例正常脑组织中的表达,并结合病理特点进行分析。结果 Chk1、Chk2蛋白在各类型胶质瘤和正常脑组织中均表达,Chk1蛋白在胶质瘤中的阳性表达水平明显高于正常脑组织(P=0.027);Chk2蛋白在胶质瘤和正常脑组织中的阳性表达水平无显著性差异(P=0.173)。Chk1、Chk2蛋白表达水平在高级别胶质瘤(WHOⅢ～Ⅳ级)和低级别胶质瘤(WHOⅠ～Ⅱ级)中无明显差异(P=0.301、0.135)。Chk1和Chk2蛋白在胶质瘤中的表达呈正相关(r=0.795,P<0.001)。结论 Chk1在胶质瘤中高表达,是胶质瘤的特异性表达分子,可以作为胶质瘤治疗的靶点,Chk2与Chk1在胶质瘤中的表达呈正相关,联合灭活Chk1、Chk2来增强胶质瘤治疗敏感性非常重要。

Objective The distribution of Chkl and Chk2 protein in glioma and brain tissue were explored to evaluate the feasibility of Chkl and Chk2 genes as the target genes of gene therapy. Methods The expressions of Chkl and Chk2 genes were detected by immune-histoehemistry in the paraffin-embeded sections of 80 gliomas and 18 cases normal brain tissue. Results Chkl and Chk2 protein were widely expressed in different glioma and normal brain tissues. The positive expression rate of Chkl protein was higher in glioma than normal brain tissues significantly （ P = 0. 027 ）. There was no significant difference in expression of Chk2 protein between glioma and normal brain tissues （ P = 0. 173 ）. The positive expression rate of Chkl and Chk2 protein was no significant difference in high-grade glioma（ WHO III - IV ） and low-grade glioma（ WHO I - II ） （P = 0. 301,0. 135 ）. There was positive correlation between Chkl and Chk2 protein（ r = 0. 795, P 〈0. 001 ）. Conclusion Chkl protein was overexpressed and could be considered as the target genes of gene therapy in glioma. There was positive correlation between Chk1 and Chk2 protein, and so it was important to block the Chk1 and Chk2 genes together to increase the sensitivity to irradiation of glioma.