鸡干扰素生产条件的比较研究〈一〉

Study of Exogenous Interferons Induced in Chicken

本试验用鸡白细胞。鸡脾细胞、鸡胚成纤维细胞以Ge－132、NDV－F、PHA、PolyI:C为诱生剂，诱生外源性IFN，将鸡胚成纤维细胞—水泡性口炎病毒作鸡IFN的检测系统，对不同种细胞、不同的细胞浓度、不同的IFN诱生剂及培养方法等诸因素对IFN产生的影响进行了比较：研究了Ge-1322、NDV－F、PHA、PolyI：C在鸡胚成纤维细胞上的最佳诱生剂量；同时也进行了不同诱生剂在不同细胞上产生的IFN的理化性状研究，结果表明：（1）在Ge－132、NDV－F、PHA、PolyI：C四种诱生剂中，诱生能力差异极显著（P＜0.01），且以Ge－132诱生IFN的能力为最强，其他依次为NDV－F、PolyI：CPHA（2）鸡脾细胞和鸡白细胞产生的IFN效价高于鸡胚成纤维细胞；（3）鸡脾细胞和鸡白细胞悬浮培养比静置培养产生的IFN效价高近1倍；（4）雏鸡脾细胞比成鸡脾细胞产生的IFN效价高；（5）未免疫鸡鸡胚成纤维细胞比免疫鸡鸡胚成纤维细胞产生IFN效价高；（6）鸡胚成纤维细胞在IFN诱生剂的作用下，4h即可产生IFN，20－24H达到最高产量；（7）IFN诱生剂最佳诱生剂量；鸡NDV－F为128HAU／ml;Ge－132为70ug／mlPolyI：C为50ug／ml;

We study exogenous interferons induced by Ge-132 in chicken.The influences of different cellspecies.cell consistencies as well as interferoninducer on the production of interferon werecompared by the chicken obroblast -vesicularstomatitis virus system for assoy of chicken inter-feron.The results showed that(1)the inducing obilityof the four species of interferon inducer (Ge-132'NDV-F.PHA and Polyl:C) tested had great signifi-cant difference(P<0.01) ond Ge-132 possessed thestrongest interferon-inducing ability,the other wereNDV-F'Po1yl;C'PHA in proper order,(2)the title ofchicken spIeen cells and chicken Ieukocytes in-terferon was higher thon that of chicken fibroblastcells,(3)the title of interferon produced in suspendedCuIture wos one time higher than static culture,(4)the title of interferon produced in young chickenspleen cells was higher than in old chickenspleen cells,(5)the best inducing-dosage of NDV-F'Ge-132.Poly 1:C and PHA was 128 HAU/ml.70ug/ml.50ug/ml and 40ug/ml respedively.