结核分枝杆菌荧光定量聚合酶链反应与噬菌体生物扩增法早期诊断结核性渗出性胸膜炎的应用价值

目的:探讨荧光定量聚合酶链反应检测技术与噬菌体生物扩增法在早期诊断结核性渗出性胸膜炎的临床应用价值。方法:对145例结核性渗出性胸膜炎患者行胸腔穿刺抽液术,标本送检结核分枝杆菌的荧光定量聚合酶链反应、噬菌体生物扩增法检测,并对两种方法进行比较。结果:荧光定量聚合酶链反应检测阳性率53.1%,高于噬菌体生物扩增法32.4%,P<0.01有极显著差异,聚合酶链反应检测明显优于噬菌体生物扩增法。结论:结核分枝杆菌的荧光定量聚合酶链反应对早期诊断结核性渗出性胸膜炎有重要的诊断价值;噬菌体生物扩增法有一定参考价值。

Objective： The objective of this paper is to discuss the clinical application value of fluorescence quantitative PCR and phage amplified biologically assay in the early diagnosis of tuberculous exudative pleurisy. Methods ： 145 tuberculous exudative pleurisy patients were operated paracentesis. The samples were detected by mycobacterium tuberculosis fluorescence quantitative PCR and phage amplified biologically assay. Then the two methods were compared. Resuits：The positive rate in fluorescence quantitative PCR was 53. 1%, higher than 32. 4% in phage amplified biologically assay, P 〈 0. 01, with a significant difference, which shows fluorescence quantitative PCR is apparently better than phage amplified biologically assay. Conclusion： Mycobacterium tuberculosis fluorescence quantitative PCR is of great diagnostic value in the early diagnosis of tuberculous exudative pleurisy, and phage amplified biologically assay is of certain reference value.